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肺基因组测序（如NGS）被认为是优选的检测方法，以一次性获取多种生物标志物信息。</li>
<li><strong>ESMO指南：</strong> ESMO指南同样推荐对所有晚期NSCLC cellpadding="5" cellspacing="0" style="width:100%">
<th style="background:#1890ff;color:#fff;padding:15px 12px;text-align:left;font-weight:600;border-right:2px solid #0d7ae6;">
<tr>
<th style="background:#1890ff;color:#fff;padding:15px 12px;text-align:left;font-weight:600;border-right:2px solid #0d7ae6;">基因</th>
<th style="background:#1890ff;color:#fff;padding:15px 12px;text-align:left;font-weight:600;border-right:2px solid #0d7ae6;">常见变异类型</th>
<th style="background:#1890ff;color:#fff;padding:15px 12px;text-align:left;font-weight:600;border-right:2px solid #0d7ae6;">靶向药物（示例）</th>
<th style="background:#1890ff;color:#fff;padding:15px 12px;text-align:left;font-weight:600;border-right:2px solid #0d7ae6;">临床适应症（示例）</th>
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</thead>
<tbody>
<tr>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">EGFR</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">外显子19缺失、L858R、T790M、外显子20曲替尼、劳拉替尼</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">ROS1阳性NSCLC一线/二线治疗</td>
</tr>
<tr>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">BRAF</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">V600E点突变</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">达拉非尼+曲美替尼</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">BRAF V600E突变阳性NSCLC</td>
</tr>
<tr>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">MET</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">外显子14跳跃突变、基因扩增</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">卡马替尼、特泊替尼</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">MET外显子14跳跃突变NSCLC</td>
</tr>
<tr>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">RET</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">基因融合（如KIF5B-RET）</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">普拉替尼、塞普替尼</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">RET融合阳性NSCLC</td>
</tr>
<tr>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">ERBB2
</tr>
<tr>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">KRAS</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">G12C点突变</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">索托拉西布、阿达格拉西布</td>
<td style="padding:12px;border-bottom:2px solid #d9d9d9;border-right:2px solid #d9d9d9;line-height:1.6;">KRAS G12C突变NS优点是快速、成本相对较低，但缺点是每次只能检测有限的位点，对于未知突变或多个基因的检测效率低下，容易漏检。例如，常规PCR只能检测EGFR的常见突变，对于
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